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If you have symptoms and believe you may have NSF a simple test to have is a punch biopsy.  A punch biopsy is performed by a dermatologist in his office.  The tissue is usually taken from the afflicated area.  Typically, reticular dermal bundles of collagen are observed with spindle cells staining positive for both CD34 and procollagen. This CD34+ immunostaining profile is actually characteristic of ‘circulating fibrocytes’, i.e. circulating cells of bone marrow origin expressing markers of both connective tissue cells and circulating leucocytes.  Mucin deposition is often seen between the collagen bundles but inflammatory cells are usually absent.  Below is a slide of a punch biopsy indicating NSF.

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